Summer school “Basics to Work With Extracellular Vesicles"
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 Export to Your Calendar 5/11/2020 to 5/13/2020
When: Monday, May 11, 2020
Where: Tartu
Estonia

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Dear readers,
 
We are pleased to invite you to the summer school “Basics to Work With Extracellular Vesicles“ that will take place on May 11-13, 2020 in Tartu, Ravila 14B.

Summer School aims to introduce the basic methods for working with Extracellular Vesicles (EVs). The EV Summer School will go through all the steps to work with EVs starting from sample preparation and EV purification until the data analysis of different EV quality and quantity data. A series of seminars and hands-on training will be provided, thus a participant would have a thorough overview of different methods applicable at UT. The seminars and hands-on training will be held by researchers and PhD students working on different EV projects under the ERA Chair of Translational Genomics at the University of Tartu. Additionally, at least two experts from outside the UT will be involved. The outcome of this Summer School would be knowledge, which helps a student to plan and conduct a project to study EVs. 
 
Summer School is open to 16 PhD / MSc students and researchers.
Completed course provides 1 ECTS credit point.
All participants need to prepare a 3-minute presentation on their current or future research project.
 
Participation is free of charge but application is required.
Letters of application with CV and motivational letter are expected until 1st of April.
 
For registration and additional information please contact oliivika@ut.ee

Program:

 

Day 1 (May 11th): EV purification

9.00-9.20

Welcoming and Introduction

Alireza Fazeli

9.20-10.45

Lecture 1

Minimum requirements for publishing EV studies

Sample preparation - Janeli Viil

10.45-11.00

Tea & Coffee break

11.00-12.00

Lecture 2

Methods for EV purification –

Getnet Midekessa & Danilo Mladenoviċ

12.00-12.40

Lunch

12.40-13:10

Student 3-minute presentations

13.10-15.10

Workshop 1

Introduction to Size Exclusion-High PerformanceLiquid Chromatography (SEC-HPLC) and Tangential Flow Filtration (TFF) -

Getnet Midekessa, Danilo Mladenoviċ & Keerthie Dissanayake

15.10-15.20

Tea & Coffee break

15.20-17.30

Workshop 2

EV purification from follicular fluid samples -

Mehedi Hasan and Qurat Ul Ain Reshi

Day 2 (May 12th): Nanoparticle Tracking Analysis (NTA)

9.00-10.00

Lecture 3

Introduction to Zetaview NTA - Sascha Raschke

Workshop 3

EV size and concentration measurements with NTA - Keerthie Dissanayake

10.00-13.00

11.00 - … Tea & Coffee break

Workshop 4

EV zeta-potential measurements with NTA -

Getnet Midekessa

13.00-13.40

Lunch

13.40-14.10

Student 3-minute presentations

14.10-16.10

Workshop 5

NTA data analysis - Freddy Lättekivi

16.10-16.20

Tea & Coffee break

16.20-17.30

Lecture 4+ Workshop 6

Olavi Reinsalu

Day 3 (May 13th): EV validation methods and further applications

9.00-10.15

Lecture 5

Transmission Electron Microscopy (TEM) - Aneta Andronowska

10.15-11.30

Lecture 6

High-resolution microscopy and its potential applications in EV research - Sergei Kopantšuk

11.30-11.40

Tea & Coffee break

11.40-12.10

Lecture 7

RNA extraction from EVs - Kasun Godakumara

12.10-13.00

Workshop 7 (part 1)

RNA extraction from EVs - Kasun Godakumara

13.00-13.40

Lunch

13.40-17.00

Workshop 7 (part 2)

RNA extraction from EVs - Kasun Godakumara

 

The course is conducted by the ERA Chair of Translational Genomics at the University of Tartu (European Union’s Horizon 2020 research and innovation programme under grant agreement No 668989).


The International Society For Extracellular Vesicles (ISEV) is a professional society of researchers and scientists of exosomes and microvesicles.

Our Mission: Advancing extracellular vesicle research globally.

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Mount Royal, New Jersey 08061
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Phone: 856-423-1621
Email: contact@isev.org

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