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"Blood EVs" is an ISEV Workshop on all things related to extracellular vesicles of blood: how to obtain, profile, and use them. The event will be held in Helsinki, Finland, from 1-2 September 2022 and will be preceded by an Education Day on 31 August. The Workshop will be in-person only, and participants have already been selected and notified. However, there will be a virtual option for Education Day. If you would like to participate virtually in the Education Day, please register here: https://www.surveymonkey.com/r/BloodEducationDay Download the full program or view below. Short talks and roundtable discussions will revolve around four themes: Day 1. Blood EVs as biomarkers
Day 2. Blood EVs as therapeutics
Only ISEV members may attend the Workshop; if you have been invited and are not a member, please visit https://www.isev.org/page/Membership to sign up. A poll on EV practices will be sent out in advance of the meeting. Workshop Chairs and Organizing Committee Full Program: ISEV WORKSHOP – Blood EVs 9:00-9.15 General welcome to the Educational day & Blood EV workshop (Saara Laitinen, Finnish Red Cross Blood Service & Pia Siljander, University of Helsinki) 9:15-9:45 Introduction to blood EVs (Rienk Nieuwland, Academic Medical Centre Amsterdam) 9:45-10:15 Blood as a source of EVs (Edit Buzas, Semmelweis University) 10:15- 10.45 Detection methods for EVs in blood (Ken Witwer, John Hopkins University) 10:45-11:10 BREAK 11:10-11:40 Pre-analytical considerations for blood collection and processing in translational and clinical EV research (Fabrice Lucien-Matteoni, Mayo Clinic) 11:40- 12:10 Clinically-relevant blood EV biomarkers – where are we? (Lorraine O’Driscoll, Trinity Collage) 12:10-13:00 Company presentations x 5 14.00-14:30 Reference values of blood EVs (Metka Lenassi, University of Ljubljana) 14:30-15:00 Approaching clinical feasibility with EV flow cytometry (Edwin van der Pol, Academic Medical Centre Amsterdam) 15:00- 15:10 Company presentation 15:10-15:30 BREAK 15:30-16.30 Heterogeneous platelet-EVs: theranostic opportunities? (Pia Siljander, University of Helsinki) 16:30-17:00 Post-isolation modifications of EVs for therapeutic purposes (Minh Le, National University of Singapore) 17:00-17:30 Summary and discussion (Rienk Nieuwland, Academic Medical Centre Amsterdam) 17:30-18:00 Results of the attendant survey & the blood EV analyses by participating technologies 19.00 Welcome reception at the Botanic gardens sponsored by Finnish Red Cross Blood Service and HansaBioMed Life Sciences DAY 1 Program Blood EVs – matrix prerequisites & technologies & biomarkers Thursday 1.9.2022 Moderators Pia Siljander (University of Helsinki) & Ken Witwer (John Hopkins University) & Lorraine O’Driscoll (Trinity Collage) 9:00-9:10 Introduction to working in the workshops, Pia Siljander 9:10-9:25 Introduction to the topic: Blood as a source of EVs Part I (Ken Witwer, John Hopkins University) 9:25 Comparison of collection tubes and processing intervals for extracellular vesicle analysis in blood (An Hendrix, Ghent University) 9:35 Influence of isolation and anticoagulant on analysis of plasma-derived EVs by imaging flow cytometry (Tobias Tertel, University Hospital Essen) 9:45 Characterization of platelet-derived extracellular vesicle subpopulations (Johanna Puutio, University of Helsinki) 9:55 Plasma EV ID: Fluorescence-based phenotyping of blood-borne extracellular vesicles (Danilo Mladenovic, Tallin University) 10:05-10:20 questions 5 minutes break 10:25 Label-free detection and characterization of single sub-micrometer particles in plasma of prostate cancer patients (Agustin Enciso-Martinez, Leiden University Medical Center) 10:35 Stratification of Breast cancer risk by Raman Spectroscopy characterization of Extracellular Vesicles (Lorena Signati, University of Milan) 10:45 Optimization of mutational analysis workflow using next-generation sequencing on plasma derived extracellular vesicles (Rebekka van Hoof, Flemish Institute for Technological Research) 10:55-11:10 questions 11:10-11:15 Break and organization into three roundtables 11:15-12:30 Roundtable discussions (60’+ 15’ wrap-up in writing by the moderators) Topics will raise organically from the participants/ electronic platform or by the chairs, for example
Table 1 Moderators: Rienk Nieuwland/Pia Siljander Table 2 Moderators: John Nolan/Edwin van der Pol Table 3 Moderators: Ann Hendrix/Metka Lenassi LUNCH 12:30-13:30 13:30-14:00 Highlights from the morning discussions by roundtable moderators (3 x 5 minutes & audience) 14:00-14:15 Introduction to the topic: Biomarker applications of the blood EV surface Part I (Lorraine O’Driscoll, Trinity Collage) 14:10 Blood EV protein corona: the opening of a new biomarker discovery landscape? (Annalisa Radeghieri, University of Brescia/CSGI, Florence) 14:20 Tumour-derived DNA is enriched as short fragments associated with extracellular vesicles (Daniel Hagey, Karolinska Institutet) 14:30 The HLA-I immunopeptidome of blood EV -detection of clinically relevant peptides (Simon Powis, University of St. Andrews) 14:40-14:55 Questions 14:55-15:25 COFFEE Biomarker applications of the blood EV surface Part II 15:25 Methods comparison for plasma-derived EVs isolation to perform proteomics analysis (Sandrine Reymond, University of Geneva) 15:35 Identification of B-cell signatures in plasma EVs from pediatric BCP-ALL patients (Marit Inngjerdingen, Oslo University Hospital) 15:45 Circulating extracellular vesicles provide valuable protein biomarkers in metastatic breast cancer (Lorena Martin-Jaular, Institut Curie) 15.45-16:00 questions 10 minutes break & moving to roundtables 16:10-17:25 Roundtable discussions (60’+ 15’ wrap-up in writing by the moderators) Topics will raise organically from the participants/ electronic platform or by the chairs Table 1 Moderators Lorraine O’Driscoll/Natasha Zavroni Table 2 Moderators Annalisa Radeghieri/Mari Palviainen Table3 Moderators Ken Witwer/Fabrice Lucien-Matteoni 5 minutes break & moving to main hall 17:30-18:00 Highlights shared from the afternoon discussions (3 x 5 min & audience) ISEV WORKSHOP on BLOOD EVs DAY 2 Program Blood EVs as Therapeutics Friday 2.9.2022 Moderators Minh Le & Saara Laitinen 9.00-9.30 Introduction to topics: Blood as a therapeutic EV source and in delivery of EV-therapy Standing on the shoulders of giants, decades of transfusion & blood cell therapy (Saara Laitinen, Finnish Red Cross Blood Service) Potential therapeutic applications of blood cell derived EVs (Minh Le, National University of Singapore) 9.30 Using plasma derived EVs as drug delivery vehicles (Maria Chiara Ferri, University of Genova) 9.40 Delivery of therapeutic mRNA via extracellular vesicles to express soluble proteins (Muhammed Nawaz, University of Gothenburg) 9.50 Development of designed targeting peptides for EV (Sujaan Das, Crane Biosciences) 10-10:15 questions & discussion 10.15 Developing functional RBC based drug delivery (Diana Piedrahita, Erytech Pharma) 10.25 Characterising safety and efficacy of RBC-EV as therapeutic delivery vehicles for retinal degenerations (Rakshanya Sekar, Australia National University) 10.35 Using RBC-EVs as drug delivery particles (Don Haut, Carmine Therapeutics) 10:45- 11:00 questions 11.00-11.15 Break and organization into three roundtables Suggestions for roundtable discussion topics in addition to those which are raised organically
11.15-13.00 Roundtable discussions Roundtable 1 Red blood cell -derived nanobiotechnology Moderators Minh Le & Kai Härkönen Roundtable 2 Different blood cell derived EVs for therapy development Moderators Saara Laitinen & Ulla Impola Roundtable 3 Lessons learned from blood EVs: is there advantage? Moderators Edith Buzas & Pia Siljander LUNCH 13.00 – 14.00 14.00-15.00 Wrap-up the Roundtables (Big hall) 15.00 Coffee 15.30-17:00 Summary of the Workshop Days 1 & 2 Moderators: Saara Laitinen & Pia Siljander & Rienk Nieuwland Wrap up of the three days into deliverables
What should we have discussed and what next?
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