Reproduction Task Force

Upcoming Events

The ISEV Rigor and Standardization subcommittee is happy to invite you to:

First virtual meeting of the ISEV Reproduction Task Force
November 10th 2022, 5pm to 8pm (CET)
    
       

Meeting is open to everyone - learn more and register here by November 1st 2022.

The goal of the EV Reproduction task force is to discuss and establish guidelines for sample collection, preparation, isolation, storage and characterization from multiple reproductive biological sources, highlighting species-specific challenges/modifications. Therefore, in this first meeting, the reproduction EV task force aims to discuss these topics with participants working on different aspects of reproduction, both basic and applied science, spanning different mammalian species.

Program:

  • 5:00 - 5:15 pm (CET) - Introduction to ISEV R&S Task forces - Juan Falcón Pérez
  • 5:15 - 6:00 pm (CET) - Introduction to the repro task force + live questionnaire using zoom pool + presenting results from a meta-analysis about the different methods for isolation/characterization/ experimentation used for repro EVs research
  • 6:00 - 7:00 pm (CET) - Methods for best practice on EVs research and most common methods used for EVs isolation and characterization - Prof. Dr. An Hendrix (University Ghent)
  • 7:00 - 7:45 pm (CET) - Breakout rooms to discuss the challenges of repro EVs research and set priorities for our task force.
  • 7:45 to 8 pm (CET) - wrap up and new member recruitment.

We are looking forward to your participation in this exciting initiative.

Marcia Ferraz
Beatriz Fernandez-Fuertes
Juliano Silveira
on behalf of ISEV Repro task force


About

The interest in the role of extracellular vesicles (EVs) in different aspects of reproduction has rapidly grown over the last decade, as evidenced by an increase in the number of publications. Due to its natural complexity (species, reproductive organ and fluid volumes) this area of research needs to be treated with caution to avoid misleading conclusions. This variety of species, fluids - which come in different volumes (from a few ul to liters), concentration of EVs, as well as culture and collection methods used, make it difficult to interpret and integrate available data. Thus, the Reproduction task force's goal is to create a set of guidelines for sample collection, preparation, isolation, storage and characterization from multiple biological sources (follicular fluid, uterine fluid, oviductal fluid, seminal plasma, gametes and embryo conditioned media) highlighting species-specific challenges/modifications. For applied EV experiments, we also want to focus on validation, toxicity and traceability issues. Additionally, we want to create a repository for antibody information, in order to help EVs scientists to avoid wasting time searching for EVs protein markers with non-validated antibodies to the species of interest.


Contact Info

Dr. Beatriz Fernandez-Fuertes ([email protected])
Dr. Juliano da Silveira ([email protected])
Dr. Marcia de Almeida Monteiro Melo Ferraz ([email protected])


Activities

  1. Determine the current state of reproductive EV research by elaborating a questionnaire that will be sent to researchers working in this field.
  2. Map researchers working with EVs in different aspects of reproduction and different countries/continents, to provide a more ample network of researchers that can lead to collaborations, exchange of protocols and ideas, as well as training opportunities.
  3. Organize a virtual symposium to discuss the results of the questionnaire, recruit contributing members and plan a positional manuscript.
  4. Write a positional manuscript(s) to help guide future experiments and make data between studies comparable.
  5. Create an antibody database for EV markers in different species