Solid Tissue Task Force

About

To date, most analyses on the role of EVs have relied on studying vesicles from fluid, such as conditioned cell culture media, or body fluids. More recently methods have developed to isolate EVs from solid tissues such as brain and tumor tissue. Relative to the routine isolation of exosomes from extracellular fluids, such as conditioned cell culture media, blood, urine or CSF, many technical issues must be overcome to limit co-isolation of vesicles with the same physical and/or molecular properties as EVs. This taskforce has been formed to help share methodology, review current practices and develop guidelines for the isolation, characterization and study of EV isolated from solid tissue.

Working Groups

N/A

Chair Contact Info

Members

Rossella Crescitelli | Laura Vella | Efrat Levy | An Hendrix | Andy Hill | Yiyao Huang | Stephanie Hurwitz | Berta Puig-Martorell | Ikezu Tsuneya

Publications

• Recommendations for Studying In Situ Extracellular Vesicles From Solid Tissue, JEV 2025. The paper is a position statement on appropriate means for separating, characterizing and reporting EVs from solid tissue. Output completed with the involvement of all TF members.

Other Outputs

• Roundtable meeting. Satellite event at ISEV2026 in San Juan (Portorico), Rossella Crescitelli, Yiyao Huang, Ikezu Tzuneya, Lucia Paolini (24 April 2026)

• Roundtable meeting. Satellite event at ISEV2025 in Vienna (Austria), Rossella Crescitelli, Lucia Paolini (24 April 2025)

• Presentation of the TF and related activities at the Rigor & Standardization session of ISEV 2024, Rossella Crescitelli (May 2024)

• Laura Vella moderated the “EVs in tissue function” session at ISEV 2024 in Melbourne (May 2024)

• Task Force member meeting at ISEV2024 annual meeting in Melbourne (May 2024)

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