2nd Virtual Symposium: Urinary EVs: 15-16 February, 2023
uEV2023 is the second Virtual Symposium on Urinary Extracellular Vesicles. Organized by the Urine Task Force of the ISEV Rigor and Standardization Subcommittee, the meeting brings together the global community studying extracellular vesicles in urine. This virtual symposium features state of the art presentations, technical updates from Urine Task Force working groups, interactive panel discussions and poster presentations. The meeting emphasizes trainee opportunities and builds upon the welcoming environment and lively discussions of uEV2022. The uEV2023 is a must-attend for nephrologists, urologists, cell and molecular biologists, biobank experts, and anyone studying urinary extracellular vesicles.
Read essential details below and then visit the full event website for complete details.
Registration
To register, use the online registration form linked below to sign in with your ISEV login or create a new account. This is a virtual meeting.
Abstracts
The deadline for abstract submission was 1 December 2022. Posters will be made available from February 14-17.
Program
*All times are CET
Program Day 1 – February 15th 2023
13:30 Welcome from the IOC 2023
Session 1: Emerging technologies/methodologies for uEV isolation, quantification, and profiling
13:40 Chairs: Fabrice Lucien and Robert Myette
13:45 Plenary Talk: David Greening, PhD, Department of Biochemistry and Genetics, La Trobe University, Australia, Integrating technologies for uEV proteome analysis
Featured abstracts
14:10 Xiao-Xiao Zhu, China, Identification of stable reference genes for relative quantification of long RNA expression in urinary exosomes
14:25 Yohan Kim, USA, Automation of clinical urinary extracellular vesicle measurement using standardized and calibrated nanoscale flow cytometry
14:40 Cristina Grange, Italy, Super resolution microscopy for single EV evaluation: better understanding of urinary EV subpopulations
14:55 Beatriz Martín-Gracia, Norway, Ultrasensitive detection of urinary prostate cancer EVs using gold nanoprisms as thermal transductors in lateral flow immunoassays
15:10 Panel Discussion: Are EV technologies ready for the clinic?
Moderator: Fabrice Lucien. Panelists: Leonora Balaj (USA), Edwin van der Pol (Netherlands), Shannon Stott (USA)
15:40 00:30 BREAK
Session 2: Urinary EV collection, handling and storage
16:10 Chairs: Maija Puhka and Martin Van Royen
16:15 Plenary Talk: Elena Martens-Uzunova, Department of Urology, Rotterdam, The Netherlands, Quality collection and biobanking for urinary EVs
Featured Abstracts
16:40 Karina Barreiro, Finland, Capturing the kidney transcriptome by urinary EV – impact of cryostorage temperature and EV isolation
16:55 Adele Tanzi, Italy, Urinary Extracellular vesicle preservation using a non-toxic dialdehyde fixative
17:10 Jenni Karttunen, Finland, Identification of the most stable and vulnerable – Impact of preanalytical variables on the uEV miRNome
17:25 Andres Lõhmus, Estonia, Tangential-flow filtration provides an easy and robust method for the isolation of urinary EVs with high yield and purity index
17:40 Introduction of the Keynote: Maija Puhka
17:45 Keynote: Prof. Per-Henrik Groop, MD, DMSc, Professor of Internal Medicine, Department of Nephrology, and Tiinamaija Tuomi, MD, PhD, Associate professor, Institute for Molecular Medicine Finland FIMM, University of Helsinki. Considerations for long-term biomarker studies – focus on diabetic kidney disease
18:30 Panel Discussion: How to improve the quality of uEV research?
Moderator: Maija Puhka. Panelists: Peter Yuen, Elena Martens-Uzunova, Per-Henrik Groop, Tiinamaija Tuomi
19:00 Closing Day 1: IOC 2023
Day 2 – February 16th 2023
13:30 Welcome from the IOC 2023 – Wrap up Day 1
Session 3: Roles of urinary EVs in (patho)physiology
13:40 Update from the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV: looking ahead (Uta Erdbruegger and Metka Lenassi)
13:50 Chairs: Cristina Grange and Per Svenningsen
13:55 Plenary Talk: Laura Perin, Division of Urology, University of Southern California, USA. In vitro and in vivo model to study kidney disease
Featured abstracts
14:20 Song-Tao Feng, China, Profiling long RNA transcriptome and tracking cell origin for urinary extracellular vesicles
14:35 Samantha Upson, USA, Daily variability of urinary extracellular vesicles in healthy subjects
14:50 Qian Chen, UK, Characterisation of urine small extracellular vesicles from normal pregnancy and early-onset preeclampsia
15:05 Danilo Bondi, Italy, Biophysical and biomolecular uEVs signatures distinguish inactive versus endurance-trained male adults
15:20 Panel Discussion: Are uEVs waste or functional?
Moderator: Benedetta Bussolati. Panelists: Lin-Li Lv, Ewout Hoorn, Per Svenningsen, Kenneth Witwer
15:50 BREAK
Session 4: Urinary EV as biomarkers in human health and disease
Chairs: Janne Leivo and Uta Erdbruegger
16:25 Plenary Talk: Francesc Enric Borras Serres IGTP and Nephrology Department, University Hospital Germans Trias i Pujol (HUGTiP), Spain. Urine Biomarker discovery for kidney fibrosis: a long & winding road
Featured abstracts
16:50 Amanda Khoo, Canada, Proteomic characterization of uEVs identifies uEV-specific protein signatures for improved risk stratification in localized prostate cancer
17:05 Mei He, USA, Isolation and Characterization of Urinary Extracellular Vesicles from Bladder Cancer Patients
17:20 Irene Bijnsdorp, The Netherlands, Detecting prostate cancer in urinary EVs making use of the surface proteome of (cancer) EVs
17:35 Loic Steiner, Sweden, The immunomodulatory role of Extracellular Vesicles in Urinary Bladder Cancer and their use as a clinical prognostic tool
17:50 Introduction of the Keynote: Fabrice Lucien
17:55 Keynote: Prof. Johan Skog, USA, Chief Scientific Officer and Vice-President at ExosomeDx. Exosome biomarkers: from bench to bedside
18:40 Panel Discussion: Which EV cargo is the better biomarker?
Moderator: Janne Leivo. Panelists: Johan Skog, Dylan Burger, Irene Binjnsdorp, Juan Falcón-Pérez
19:10 Closing Day 2: IOC 2023 and Award ceremony
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