2nd Virtual Symposium: Urinary EVs: 15-16 February, 2023uEV2023 is the second Virtual Symposium on Urinary Extracellular Vesicles. Organized by the Urine Task Force of the ISEV Rigor and Standardization Subcommittee, the meeting brings together the global community studying extracellular vesicles in urine. This virtual symposium features state of the art presentations, technical updates from Urine Task Force working groups, interactive panel discussions and poster presentations. The meeting emphasizes trainee opportunities and builds upon the welcoming environment and lively discussions of uEV2022. The uEV2023 is a must-attend for nephrologists, urologists, cell and molecular biologists, biobank experts, and anyone studying urinary extracellular vesicles. Read essential details below and then visit the full event website for complete details. Visit Event WebsiteRegistrationTo register, use the online registration form linked below to sign in with your ISEV login or create a new account. This is a virtual meeting. RegisterAbstractsThe deadline for abstract submission was 1 December 2022. Posters will be made available from February 14-17. Program*All times are CET Program Day 1 – February 15th 202313:30 Welcome from the IOC 2023 Session 1: Emerging technologies/methodologies for uEV isolation, quantification, and profiling 13:40 Chairs: Fabrice Lucien and Robert Myette 13:45 Plenary Talk: David Greening, PhD, Department of Biochemistry and Genetics, La Trobe University, Australia, Integrating technologies for uEV proteome analysis Featured abstracts 14:10 Xiao-Xiao Zhu, China, Identification of stable reference genes for relative quantification of long RNA expression in urinary exosomes 14:25 Yohan Kim, USA, Automation of clinical urinary extracellular vesicle measurement using standardized and calibrated nanoscale flow cytometry 14:40 Cristina Grange, Italy, Super resolution microscopy for single EV evaluation: better understanding of urinary EV subpopulations 14:55 Beatriz Martín-Gracia, Norway, Ultrasensitive detection of urinary prostate cancer EVs using gold nanoprisms as thermal transductors in lateral flow immunoassays 15:10 Panel Discussion: Are EV technologies ready for the clinic? 15:40 00:30 BREAK Session 2: Urinary EV collection, handling and storage 16:10 Chairs: Maija Puhka and Martin Van Royen 16:15 Plenary Talk: Elena Martens-Uzunova, Department of Urology, Rotterdam, The Netherlands, Quality collection and biobanking for urinary EVs Featured Abstracts 16:40 Karina Barreiro, Finland, Capturing the kidney transcriptome by urinary EV – impact of cryostorage temperature and EV isolation 16:55 Adele Tanzi, Italy, Urinary Extracellular vesicle preservation using a non-toxic dialdehyde fixative 17:10 Jenni Karttunen, Finland, Identification of the most stable and vulnerable – Impact of preanalytical variables on the uEV miRNome 17:25 Andres Lõhmus, Estonia, Tangential-flow filtration provides an easy and robust method for the isolation of urinary EVs with high yield and purity index 17:40 Introduction of the Keynote: Maija Puhka 17:45 Keynote: Prof. Per-Henrik Groop, MD, DMSc, Professor of Internal Medicine, Department of Nephrology, and Tiinamaija Tuomi, MD, PhD, Associate professor, Institute for Molecular Medicine Finland FIMM, University of Helsinki. Considerations for long-term biomarker studies – focus on diabetic kidney disease 18:30 Panel Discussion: How to improve the quality of uEV research? 19:00 Closing Day 1: IOC 2023 Day 2 – February 16th 202313:30 Welcome from the IOC 2023 – Wrap up Day 1 Session 3: Roles of urinary EVs in (patho)physiology 13:40 Update from the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV: looking ahead (Uta Erdbruegger and Metka Lenassi) 13:50 Chairs: Cristina Grange and Per Svenningsen 13:55 Plenary Talk: Laura Perin, Division of Urology, University of Southern California, USA. In vitro and in vivo model to study kidney disease Featured abstracts 14:20 Song-Tao Feng, China, Profiling long RNA transcriptome and tracking cell origin for urinary extracellular vesicles 14:35 Samantha Upson, USA, Daily variability of urinary extracellular vesicles in healthy subjects 14:50 Qian Chen, UK, Characterisation of urine small extracellular vesicles from normal pregnancy and early-onset preeclampsia 15:05 Danilo Bondi, Italy, Biophysical and biomolecular uEVs signatures distinguish inactive versus endurance-trained male adults 15:20 Panel Discussion: Are uEVs waste or functional? 15:50 BREAK Session 4: Urinary EV as biomarkers in human health and disease 16:25 Plenary Talk: Francesc Enric Borras Serres IGTP and Nephrology Department, University Hospital Germans Trias i Pujol (HUGTiP), Spain. Urine Biomarker discovery for kidney fibrosis: a long & winding road Featured abstracts 16:50 Amanda Khoo, Canada, Proteomic characterization of uEVs identifies uEV-specific protein signatures for improved risk stratification in localized prostate cancer 17:05 Mei He, USA, Isolation and Characterization of Urinary Extracellular Vesicles from Bladder Cancer Patients 17:20 Irene Bijnsdorp, The Netherlands, Detecting prostate cancer in urinary EVs making use of the surface proteome of (cancer) EVs 17:35 Loic Steiner, Sweden, The immunomodulatory role of Extracellular Vesicles in Urinary Bladder Cancer and their use as a clinical prognostic tool 17:50 Introduction of the Keynote: Fabrice Lucien 17:55 Keynote: Prof. Johan Skog, USA, Chief Scientific Officer and Vice-President at ExosomeDx. Exosome biomarkers: from bench to bedside 18:40 Panel Discussion: Which EV cargo is the better biomarker? 19:10 Closing Day 2: IOC 2023 and Award ceremony |